SUBMICROSCOPIC PARTICLES IN EXTRACTS OF AZOTOBACTER AGILIS

Induced biochemical mutants to Azotobacter agilis.

Metabolic Studies of Azotobacter Agilis by the Use of a Mutant Deficient in Pyruvic Oxidase*

An account has been given elsewhere of the successful isolation of physiological x-ray mutants of Azotobacter agilis with genetic blocks in the substrate oxidation mechanisms (1). The present paper deals in greater detail with a mutant strain, designated A13, that was isolated as a variant unable to grow on glucose, and gives the methods used to characterize the particular deficiency responsibl...

Coupled oxidative phosphorylation in crude extracts of Azotobacter.

Two outstanding characteristics of azotobacter, their abilitv to fix nitrogen and unusually high rates of oxidation, have stimulated interest in obtaining cell free extracts suitable for biochemical analysis of these processes. Many difficulties have been encountered in obtaining such extracts. Attempts to obtain cell free extracts capable of nitrogen fixation have given extremely inconsistent ...

The incorporation of acetate in acids of the citric acid cycle by Azotobacter extracts.

Experiments were carried out in manometric vessels of sufficient size to accommodate 18 ml. of reaction mixture. These were made up by using the same proportion of reactants as previously described (1) : 1.20 ml. of 0.2 M phosphate buffer (pH 6.89, 0.6 ml. of 0.02 M MgSOd, 0.6 ml. of 0.025 per cent coenzyme A, 0.6 ml. of 0.1 M sodium acetate-1-C14, 1.2 ml. of 0.1 M sodium citrate, and 3.0 ml. o...

Mechanism of Biological Nitrogen Fixation X. Hydrogenase in Cell-free Extracts and Intact Cells of Azotobacter*

The discovery of hydrogenase in Azotobacter cells (1) raises the question of its function in the metabolism of this organism. This enzyme is usually found in anaerobic or facultative anaerobic bacteria which liberate Hz from certain substrates. Azotobacter is a strict aerobe, and its metabolism consists primarily in the simple oxidation of substrate to carbon dioxide and water. Although not con...